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Updated: May 11, 2026

In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
Changes in CO2 concentration increase the invasive ability of colon cancer cells
Shinsuke Obata1, Takanori Goi, Toshiyuki Nakazawa
1First Department of Surgery, University of Fukui, 23-3, Eiheiji-cho, Yoshida-gun, Fukui, Japan.
Background/Aim:
We studied the effects of CO(2) concentration changes on the invasive ability of colon cancer cells.
Materials And Methods:
Colon cancer cell lines and human samples derived from a peritoneal metastasis were incubated in a hypercapnic environment, followed by incubation in 5% CO(2). The invasive ability of colon cancer cells incubated with CO(2) were analyzed using an invasion assay system.
Results:
In comparison with the colon cancer cell lines incubated in 5% CO(2) only, the invasive ability of cells increased in all the colon cancer cell lines subjected to incubation in 20% CO(2) followed by incubation in 5% CO(2), with a concomitant increase in the mRNA expressions of matrix metalloproteinase-2 (MMP2) and MMP9. The invasive capability of peritoneal metastatic cells in the human-derived specimen also increased on CO(2) concentration changes.
Conclusion:
CO(2) concentration changes enhanced the invasive capacity of colon cancer cells.
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