[Applying multiple displacement amplification to DNA typing in the pathological section].

Area of Science:

• Forensic science
• Molecular biology
• Genetics

Context:

• Forensic pathological sections are crucial for DNA typing in legal investigations.
• Degradation of DNA in preserved tissues can hinder accurate genotyping.
• Traditional PCR amplification may be insufficient for low-quantity or degraded DNA samples.

Purpose:

• To evaluate the efficacy of multiple displacement amplification (MDA) for DNA typing in forensic pathological sections.
• To compare MDA-assisted DNA typing with direct PCR amplification.
• To determine the influence of preservation period, tissue type, and age of death on MDA performance.

Summary:

• Ninety-eight pathological sections were analyzed using DNA extraction via the silicon bead method.
• Samples were divided into a control group (direct PCR) and an experimental group (MDA followed by PCR).
• MDA significantly improved DNA typing success rates, with over 95% detection for 16 loci within 360 days of preservation, outperforming direct PCR.

Impact:

• MDA is a viable technique for improving DNA template quantification in forensic pathological samples.
• MDA effectively reduces PCR inhibition and allele dropout rates, enhancing genotyping accuracy.
• Preservation duration and tissue type are critical factors influencing the success of MDA in forensic DNA typing.