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Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Updated: May 11, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

A novel electromagnetic apparatus for rapid multiplex single molecule force spectroscopy.

Yi Shen¹, Daniel M Czajkowsky, Xiaowei Li

¹Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

Journal of Nanoscience and Nanotechnology

|May 8, 2013

Summary

Researchers developed a novel electromagnetic apparatus for multiplex single molecule force measurements. This system enables rapid, simultaneous probing of multiple biomolecules, overcoming limitations in traditional single-molecule force spectroscopy.

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Last Updated: May 11, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

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Published on: July 25, 2012

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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Area of Science:

Biophysics
Nanotechnology
Biochemistry

Background:

Single-molecule force spectroscopy (SMFS) provides detailed insights into molecular interactions.
High throughput is needed to overcome statistical limitations in traditional SMFS.

Purpose of the Study:

To develop a novel electromagnetic apparatus for multiplex single-molecule force measurements.
To enable simultaneous analysis of multiple biomolecules for enhanced statistical reliability.

Main Methods:

Utilized magnetic microspheres for multiplex single-molecule force measurements.
Integrated an electron-lens from an electron microscope with a high-voltage flash circuit to generate magnetic fields.
Achieved rapid magnetic field generation (up to -20 pN within 5 ms over 1 mm).

Main Results:

Demonstrated a novel electromagnetic apparatus for multiplex SMFS.
Successfully applied the apparatus to measure force-dependent extension of double-stranded DNA (dsDNA).
Validated the instrument's capability for high-throughput, statistically reliable measurements.

Conclusions:

The developed apparatus significantly enhances throughput for SMFS.
The system's design is compatible with optical imaging, allowing integration of fluorescence capabilities.
This multi-dimensional characterization approach is powerful for studying fast molecular processes.