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Dependence between 5'-nucleotidase activity and phosphatidylcholine content in pig adipocyte plasma membrane. Absence
C Nicolas1, Y Demarne, M J Lecourtier
1Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Jouy-en-Josas, France.
Abstract:
1. 5'-Nucleotidase activity has been examined in pig adipocyte plasma membrane, in two different adipose sites. 2. Membrane fluidity has been investigated using 1,6-diphenylhexatriene (DPH). 3. In this work, evidence was found for a tissue specificity of 5'-nucleotidase (E.C. 3.1.3.5) activity. 4. The enzyme activity was highly correlated to the membrane phosphatidylcholine content. 5. The latter parameters remained unmodified when large alterations in membrane composition and physical state were produced by means of the dietary n-6 PUFA content manipulations.