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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

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Nuclear MMP-9 role in the regulation of rat skeletal myoblasts proliferation

Malgorzata Zimowska1, Marta Swierczynska, Maria A Ciemerych

  • 1Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland. mzimowska@biol.uw.edu.pl

Biology of the Cell
|May 8, 2013
PubMed
Abstract

Insights

Matrix metalloproteinases-9 (MMP-9) is found in the nuclei of differentiating myoblasts and influences their proliferation. Inhibiting MMP-9 affects its nuclear localization and myoblast cell cycle progression.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Muscle Regeneration

Background:

  • Matrix metalloproteinases (MMPs) remodel the extracellular matrix.
  • MMP-2 and MMP-9 are involved in skeletal muscle regeneration.
  • Recent findings suggest intracellular roles for MMPs.

Purpose of the Study:

  • Investigate intracellular localization of gelatinases during myoblast differentiation.
  • Determine the impact of MMP inhibition on myoblast proliferation and function.

Main Methods:

  • Studied intracellular localization of MMP-9 in differentiating myoblasts in vitro.
  • Assessed the effects of MMP inhibitors (doxycycline, TIMP-1, anti-MMP-9 antibody) on myoblast proliferation and MMP-9 nuclear localization.

Main Results:

  • MMP-9 was localized within the S-phase nuclei of differentiating myoblasts.
  • Inhibition of MMPs affected MMP-9 nuclear localization.
  • MMP inhibition impacted myoblast proliferation.

Conclusions:

  • Nuclear MMP-9 may play a role in regulating cell cycle progression during myoblast differentiation.
Keywords:
DifferentiationMMP-9MyoblastNucleusProliferation

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