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Updated: May 11, 2026
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
Published on: February 6, 2017
A simple and novel method for GII norovirus genome clone with generic primers
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong, China.
Aims:
This study aims to establish a novel method for cloning GII norovirus genome using generic primers rationally designed based on multiple alignments of 96 GII norovirus genome sequences.
Methods And Results:
Based on conservative analysis of 96 GII norovirus genome sequences available in GenBank, three fragments encompassing the full-length genome were rationally designed. Fragments A, B and C were amplified by primers N1F/N2819R, N2689F/COG2R and COG2F/adaptor, respectively. Meanwhile, the sensitivity of the novel primers was evaluated, which could achieve 10(1) RTPCRU, as determined by the common detection primer pair JV12/JV13. The availability of the novel protocol was verified by sequencing two norovirus strains with different genotypes.
Conclusions:
Primers for GII norovirus genome clone were rationally designed, and a novel GII genome clone method was established.
Significance And Impact Of The Study:
The three-fragment cloning method can be used as a universal tool to collect information on the genome of norovirus strains for future evolution and antivirus studies.
