Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic background selection with BasCoD enhances contrastive dimension reduction in single cell genomics.

Nature communications·2026
Same author

Genetic variation and inflammation intersections: GATA2- and RUNX1-linked mechanisms of blood pathogenesis.

Experimental hematology·2026
Same author

Humoral immunity induced by XEC monovalent vaccines against SARS-CoV-2 variants including XEC, LP.8.1, NB.1.8.1, XFG, and BA.3.2.

Vaccine·2026
Same author

Humoral immunity after LP.8.1 monovalent vaccines against a broad range of SARS-CoV-2 variants including XEC, LP.8.1, NB.1.8.1, XFG, and BA.3.2.

The Lancet. Infectious diseases·2026
Same author

Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms.

Nature communications·2025
Same author

GATA2 decommissioning of enhancers: A mechanism to constrain inflammatory signaling.

Cell reports·2025

Related Experiment Video

Updated: May 11, 2026

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

NF-E2 p45 is important for establishing normal function of platelets.

Rie Fujita1, Mariko Takayama-Tsujimoto, Hironori Satoh

  • 1Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan.

Molecular and Cellular Biology
|May 8, 2013
PubMed
Summary

The transcription factor NF-E2 (p45) is essential for megakaryocyte function, regulating platelet production and quality. This study identifies direct p45 targets, revealing its critical role in platelet quantity and reactivity.

More Related Videos

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Related Experiment Videos

Last Updated: May 11, 2026

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Area of Science:

  • Hematology
  • Molecular Biology
  • Gene Regulation

Background:

  • NF-E2 is a heterodimeric transcription factor crucial for platelet production.
  • p45, a subunit of NF-E2, is known to regulate megakaryocytes and platelet formation.
  • Thrombocytopenia observed in p45-deficient mice highlights its importance.

Purpose of the Study:

  • To identify direct target genes of the p45 subunit in megakaryocytes.
  • To investigate the role of p45 in regulating platelet production and function.
  • To determine how p45 influences platelet reactivity and overall hemostasis.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) sequencing to map p45 genome-wide occupancy in primary megakaryocytes.
  • Verification of direct p45 targets (e.g., Selp, Myl9) using multiple experimental approaches.
  • Analysis of platelet function and melanoma metastasis in mice with a hypomorphic p45 mutation in megakaryocytes.

Main Results:

  • ChIP sequencing identified numerous p45 target gene candidates involved in platelet production and function.
  • Selp and Myl9 were confirmed as direct p45 targets.
  • Mice with reduced p45 activity in megakaryocytes exhibited impaired platelet function and reduced melanoma metastasis, indicating compromised platelet quality.

Conclusions:

  • p45 directly regulates genes essential for both platelet production and function.
  • The transcription factor p45 dictates the quantity and intrinsic quality of platelets.
  • p45 plays a vital role in maintaining effective hemostasis and regulating platelet-dependent processes like cancer metastasis.