Related Experiment Video
Updated: May 11, 2026

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Published on: January 26, 2024
Characterization and complete genome sequence of a virulent bacteriophage B4 infecting food-borne pathogenic Bacillus
Ju-Hoon Lee1, Hakdong Shin, Bokyung Son
1Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Korea.
Abstract:
Bacillus cereus causes food poisoning, resulting in vomiting and diarrhea, due to production of enterotoxins. As a means of controlling this food-borne pathogen, the virulent bacteriophage B4 was isolated and characterized. Bacterial challenge assays showed that phage B4 effectively inhibited growth of members of the B. cereus group as well as B. subtilis, and growth inhibition persisted for over 20 h. One-step growth analysis also revealed the host lysis activity of phage B4, with relatively short eclipse/latent times (10/15 min) and a large burst size (>200 PFU). The complete genome of phage B4, containing a 162-kb DNA with 277 ORFs, was analyzed. The endolysin encoded by the phage B4 genome accounts for the cell lysis activity of this phage. These results suggest that phage B4 has potential as a biological agent to control B. cereus propagation.
More Related Videos
07:22Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
Published on: August 19, 2021
12:23Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Related Concept Videos
Viral Replication: Lytic Cycle
DNA Bacteriophages
Regulation of Bacterial Virulence
Lysogenic Cycle of Bacteriophages
Bacterial Gastroenteritis
Lytic Cycle of Bacteriophages