Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Teratogenicity01:07

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnosis of chronic pancreatitis using semi-quantitative MRI features of the pancreatic parenchyma: results from the multi-institutional MINIMAP study.

Abdominal radiology (New York)·2023
Same author

Factors Associated With Visual Acuity Decline in Glaucoma Patients With Loss of Ganglion Cell Complex Thickness.

Translational vision science & technology·2023
Same author

Light and heavy chain deposition disease with focal amyloid deposition diagnosed with mass spectrometry: a case report.

BMC nephrology·2023
Same author

Clinical Validation of Plasma-Based Genotyping for <i>RAS</i> and <i>BRAF</i> V600E Mutation in Metastatic Colorectal Cancer: SCRUM-Japan GOZILA Substudy.

JCO precision oncology·2023
Same author

A Rare Cause of Abdominal Pain: Erdheim-Chester Disease.

ACG case reports journal·2023
Same author

Feasibility of Renal Blood Flow Measurement Using <sup>64</sup>Cu-ATSM PET/MRI: A Quantitative PET and MRI Study.

Diagnostics (Basel, Switzerland)·2023

Related Experiment Video

Updated: May 11, 2026

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
10:07

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

Teratogenic factors affect transcription factor expression.

Takuya Kojima1, Shinya Asano, Naoki Takahashi

  • 1Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.

Bioscience, Biotechnology, and Biochemistry
|May 8, 2013
PubMed
Summary

Predicting teratogenic risk is crucial. This study shows that changes in developmental transcription factor gene expression, like Hox genes, can accurately predict adverse effects on fetal development.

More Related Videos

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

Related Experiment Videos

Last Updated: May 11, 2026

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
10:07

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

Area of Science:

  • Developmental biology
  • Toxicology
  • Genetics

Background:

  • Chemical compounds pose risks to human health, necessitating accurate risk prediction.
  • Teratogens severely impact fetal development, traditionally assessed via animal phenotypic analysis.
  • Species-specific phenotypic variations limit traditional teratogen assessment methods.

Purpose of the Study:

  • To establish a novel evaluation system for teratogenic risk prediction.
  • To investigate the influence of teratogens on gene expression, particularly Hox genes.
  • To identify specific developmental transcription factors altered by teratogen exposure.

Main Methods:

  • Established a new evaluation system based on teratogen-induced changes in Hox gene expression in mice.
  • Exposed mice to retinoic acid (RA) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) for 6 hours.
  • Monitored expression patterns of developmental transcription factors including Dlx, Irx, Sall, and T-box families.

Main Results:

  • Teratogens (RA, TCDD) altered the expression of developmental transcription factors within 6 hours.
  • Specific gene expression changes (Dlx4, Dlx6, Irx5, Sall2, Sall3, Sall4, Tbx10, Tbx22) were observed.
  • These expression changes correlated with teratogen-induced phenotypes.

Conclusions:

  • Changes in developmental transcription factor expression serve as reliable indicators of teratogenic risk.
  • This novel system offers a rapid and accurate method for predicting teratogenic effects.
  • Gene expression profiling provides a valuable tool for assessing chemical compound safety.