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Related Experiment Video

Updated: May 11, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

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Published on: January 8, 2014

Functional monolithic platforms: chromatographic tools for antibody purification.

Telma Barroso¹, Abid Hussain, Ana C A Roque

¹REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.

Biotechnology Journal

|May 8, 2013

Summary

Polymer monoliths offer an efficient method for antibody purification, crucial for therapeutic antibody development. This review details their use in chromatography for enhanced antibody capture and release.

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Area of Science:

Biotechnology
Materials Science
Analytical Chemistry

Background:

The therapeutic landscape is increasingly dominated by monoclonal antibodies (mAbs) and engineered antibody structures.
Efficient and scalable purification methods are essential to meet clinical and performance demands for these biologics.

Purpose of the Study:

This review comprehensively analyzes synthetic and natural polymer-based monoliths for antibody purification.
It highlights advancements in materials, production methods, characterization, and chromatographic performance.

Main Methods:

Discussion of various synthetic and natural polymer materials used in monolith fabrication.
Review of methods for monolith production and structural characterization.
Analysis of chromatographic techniques for antibody capture and release using monolithic platforms.

Main Results:

Polymer monoliths provide practical advantages including faster analysis, reduced sample volumes, and increased target molecule enrichment.
Different monolithic systems exhibit unique properties impacting antibody purification efficiency.
Successful application of monoliths in various antibody separation strategies has been demonstrated.

Conclusions:

Polymer monoliths represent a remarkable and versatile platform for antibody purification.
Ongoing research trends aim to address current and future challenges in antibody separation technology.