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Related Experiment Video

Updated: May 11, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

MAP kinases bind endothelial nitric oxide synthase.

Carol A Chrestensen1, Jonathan L McMurry, John C Salerno

  • 1Department of Chemistry & Biochemistry, Kennesaw State University, Kennesaw, GA 30144-1203, USA.

FEBS Open Bio
|May 8, 2013
PubMed
Summary

Endothelial nitric oxide synthase (eNOS) directly binds mitogen-activated protein kinases (MAPKs) like p38 and ERK. This interaction, modulated by calmodulin, suggests MAPKs regulate NOS activity, impacting vascular tone and angiogenesis.

Keywords:
AI, autoinhibitory element of nitric oxide synthaseATF, activating transcription factorAkt, v-akt murine thymoma viral oncogene homolog 1 (a.k.a, protein kinase B)BAEC, bovine aortic endothelial cellsCaM, calmodulinERKERK1/2, mitogen activated protein kinase 1 and 2MAP kinaseMEF, myocyte enhancer factorMK or MAPKAP kinase, mitogen activated protein kinase activated protein kinaseNitric oxide synthaseOptical biosensingPKA, protein kinase AeNOS, endothelial nitric oxide synthasenNOS, neuronal nitric oxide synthasep38

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07:13

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Published on: January 7, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Signaling

Background:

  • Endothelial nitric oxide synthase (eNOS) is crucial for vascular homeostasis.
  • Mitogen-activated protein kinases (MAPKs) are key regulators of cellular processes.
  • The interaction between eNOS and MAPKs has not been fully elucidated.

Purpose of the Study:

  • To investigate the direct binding interactions between eNOS and specific MAPKs (p38 and ERK).
  • To characterize the kinetics and affinity of these binding events.
  • To explore the role of calmodulin in modulating p38-eNOS interactions.

Main Methods:

  • Utilized optical biosensing to measure binding kinetics and affinity.
  • Employed calmodulin titration to assess its effect on p38-eNOS binding.
  • Investigated the formation of ternary complexes.

Main Results:

  • eNOS directly bound p38 and ERK with nanomolar affinity and complex kinetics.
  • Binding was found to be diffusion-limited.
  • Neuronal NOS showed weaker binding to p38.
  • Calmodulin inhibited p38-eNOS binding and promoted dissociation, suggesting ternary complex formation.

Conclusions:

  • MAPKs, particularly p38, directly interact with and likely regulate eNOS activity.
  • Calmodulin plays a role in modulating this interaction.
  • These findings have significant implications for understanding signaling pathways in angiogenesis and vascular tone regulation.