Related Experiment Video
Updated: May 11, 2026

CRISPR-Mediated Reorganization of Chromatin Loop Structure
Published on: September 14, 2018
High-resolution analysis of cis-acting regulatory networks at the α-globin locus
Jim R Hughes1, Karen M Lower, Ian Dunham
1MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford OX3 9DS, UK.
Abstract:
We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.
Related Concept Videos
Cis-regulatory Sequences
Cis-regulatory Sequences
General Transcription Factors
Master Transcription Regulators

