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Related Experiment Video

Updated: May 11, 2026

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Published on: November 12, 2013

NeuroD regulates neuronal migration.

Woo-Young Kim¹

¹Department of Molecular, Cellular and Developmental Biology, University of Colorado, Aurora, CO, 80309, USA. wykim@sookmyung.ac.kr

Molecules and Cells

|May 9, 2013

Summary

This summary is machine-generated.

NeuroD is crucial for neuron survival and migration. Deleting the Bax gene rescues NeuroD-deficient neuronal death via apoptosis, highlighting NeuroD

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Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

NeuroD is essential for vertebrate central nervous system neuron survival.
NeuroD deficiency causes premature neuronal death, obscuring its role in later neurogenesis stages.
The mechanism of NeuroD-deficient neuronal death remains uninvestigated.

Purpose of the Study:

To investigate the role of NeuroD in neuronal survival during later neurogenesis stages.
To elucidate the mechanism of NeuroD-deficient neuronal death.
To test the hypothesis that NeuroD-dependent neuronal death occurs via a Bax-dependent apoptotic pathway.

Main Methods:

Generation of NeuroD and Bax double null mice.
Analysis of neuronal apoptosis in the hippocampus and cerebellum.
Assessment of vestibulocochlear ganglion (VCG) neuron migration and innervation.
Evaluation of TrkB and TrkC expression in VCG neurons.

Main Results:

NeuroD and Bax double null mice showed reduced apoptosis in the hippocampus and cerebellum.
VCG neuron migration and innervation were rescued in double null mice.
NeuroD and Bax double null VCG neurons failed to express TrkB and TrkC at E13.5.

Conclusions:

Neuronal death in NeuroD null mice is mediated by Bax-dependent apoptosis.
NeuroD is required for VCG neuron migration and innervation.
NeuroD is necessary for TrkB and TrkC expression, which are vital for VCG neuron migration and innervation.