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Anti-senescence efficacy of radio-electric asymmetric conveyer technology
Margherita Maioli1, Salvatore Rinaldi, Sara Santaniello
1Department of Biomedical Sciences, University of Sassari, Viale San Pietro, 43/B, 07100, Sassari, Italy.
Abstract:
Recent evidence suggests that ageing-related diseases could result in an accelerated loss of self-renewal capability of adult stem cells, normally involved in replacing damaged cellular elements. In previous works, we highlighted that a specific treatment, named tissue optimization-regenerative (TO-RGN), of radio-electric asymmetric conveyer (REAC) technology, influenced gene expression profiles controlling stem cell differentiation and pluripotency of human skin-derived fibroblasts in vitro. The purpose of the present work was to verify whether TO-RGN may also be effective in counteracting the expression of the senescence marker beta-galactosidase and of senescence-associated gene expression patterning, engaged during prolonged culture of human adipose-derived stem cells (hADSCs). Following TO-RGN exposure, we observed a significant downregulation in beta-galactosidase staining and in the expression of the senescence mediator genes p16INK4, ARF, p53, and p21(CIP1). Moreover, differently formed untreated cells, TO-RGN-exposed hADSCs maintained their typical fibroblast-like morphology and exhibited a multilineage potential even at late passages, as shown by the remarkable preservation of commitment to osteogenic, adipogenic, chondrogenic, and vasculogenic fates, both at morphologic and gene expression levels. In conclusion, our study highlights a positive effect of TO-RGN in counteracting degenerative senescence processes in vitro.
Insights
Tissue optimization-regenerative (TO-RGN) treatment counteracts cellular senescence in human adipose-derived stem cells. This regenerative medicine approach preserves stem cell function and multilineage potential, offering a promising strategy against age-related cellular decline.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Aging Research
Background:
- Ageing-related diseases accelerate adult stem cell loss, impairing tissue repair.
- Previous work showed radio-electric asymmetric conveyer (REAC) technology's tissue optimization-regenerative (TO-RGN) treatment influences stem cell pluripotency.
- The effect of TO-RGN on senescence in human adipose-derived stem cells (hADSCs) requires investigation.
Purpose of the Study:
- To evaluate if TO-RGN treatment can counteract senescence markers in hADSCs.
- To assess the impact of TO-RGN on the expression of senescence-associated genes.
- To determine if TO-RGN preserves the multilineage potential of hADSCs during prolonged culture.
Main Methods:
- Human adipose-derived stem cells (hADSCs) were exposed to TO-RGN treatment.
- Beta-galactosidase staining was used to assess senescence.
- Gene expression analysis was performed for senescence mediators (p16INK4, ARF, p53, p21(CIP1)).
- Multilineage differentiation potential (osteogenic, adipogenic, chondrogenic, vasculogenic) was evaluated morphologically and at the gene expression level.
Main Results:
- TO-RGN treatment significantly downregulated beta-galactosidase staining.
- Expression of key senescence mediator genes (p16INK4, ARF, p53, p21(CIP1)) was reduced.
- TO-RGN-exposed hADSCs maintained fibroblast-like morphology and multilineage potential at late passages.
- Preservation of osteogenic, adipogenic, chondrogenic, and vasculogenic fates was observed.
Conclusions:
- TO-RGN treatment effectively counteracts degenerative senescence processes in hADSCs in vitro.
- This regenerative approach preserves stem cell morphology and multilineage differentiation capacity.
- TO-RGN shows potential for mitigating age-related stem cell dysfunction.
