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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Phagocytosis00:41

Phagocytosis

Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.

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Related Experiment Video

Updated: May 11, 2026

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
09:43

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Published on: December 7, 2019

Teaching phagocytosis using flow cytometry.

John T Boothby1, Ruthann Kibler, Sabine Rech

  • 1Departments of Biological Sciences and.

Microbiology Education
|May 9, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces flow cytometry for teaching basic microbiology, specifically phagocytosis in Tetrahymena sp. Students found the method intuitive and effective for learning investigative microbiology.

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Visualizing the Early Stages of Phagocytosis
08:04

Visualizing the Early Stages of Phagocytosis

Published on: February 3, 2017

Related Experiment Videos

Last Updated: May 11, 2026

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
09:43

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Published on: December 7, 2019

Visualizing the Early Stages of Phagocytosis
08:04

Visualizing the Early Stages of Phagocytosis

Published on: February 3, 2017

Area of Science:

  • Microbiology
  • Cell Biology
  • Educational Technology

Background:

  • Investigative microbiology in teaching labs faces limitations in student skills, culture ease, instrumentation, and time.
  • Flow cytometry, widely used in research and clinical settings, has seen limited application in educational microbiology labs.
  • High cost and limited trained instructors hinder flow cytometry adoption in teaching environments.

Purpose of the Study:

  • To develop and evaluate a flow cytometry-based laboratory module for teaching phagocytosis in Tetrahymena sp.
  • To assess the feasibility and effectiveness of using flow cytometry in a basic microbiology teaching laboratory.
  • To gauge student and instructor perceptions of the learning experience and data analysis tools.

Main Methods:

  • Implementation of a laboratory module focused on Tetrahymena sp. phagocytosis.
  • Utilizing flow cytometry for data acquisition and analysis.
  • Employing the Paint-A-Gate (PRO-TM) software for intuitive data analysis.
  • Conducting student learning assessments using an inquiry-based format.

Main Results:

  • Students and instructors found the Paint-A-Gate (PRO-TM) software intuitive and easy to learn.
  • The flow cytometry module effectively facilitated student learning about Tetrahymena sp. and phagocytosis.
  • Inquiry-based assessments indicated a positive overall student response to the learning experience.

Conclusions:

  • Flow cytometry is a viable and effective tool for teaching basic microbiology concepts, including phagocytosis.
  • The developed laboratory module demonstrates the potential of flow cytometry in enhancing microbiology education.
  • The accessibility and ease of use of flow cytometry technology can be expanded to basic teaching laboratories.