Related Experiment Video
Updated: May 11, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Studying gene expression: database searches and promoter fusions to investigate transcriptional regulation in
Betsy M Martinez-Vaz1, Irina Makarevitch, Shane Stensland
1Department of Biology, Hamline University, Saint Paul, MN 55104.
Abstract:
A laboratory project was designed to illustrate how to search biological databases and utilize the information provided by these resources to investigate transcriptional regulation in Escherichia coli. The students searched several databases (NCBI Genomes, RegulonDB and EcoCyc) to learn about gene function, regulation, and the organization of transcriptional units. A fluorometer and GFP promoter fusions were used to obtain fluorescence data and measure changes in transcriptional activity. The class designed and performed experiments to investigate the regulation of genes necessary for biosynthesis of amino acids and how expression is affected by environmental signals and transcriptional regulators. Assessment data showed that this activity enhanced students' knowledge of databases, reporter genes and transcriptional regulation.
More Related Videos
10:18A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
Published on: April 25, 2015
13:48Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
Published on: January 18, 2020
Related Concept Videos
Coordination of Gene Expression Processes in Bacteria
Reporter Genes
Commonly used reporter...
Translational Regulation
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Constitutive and Regulated Gene Expression