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Updated: May 11, 2026

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Published on: September 8, 2023

A colorimetric method for H1N1 DNA detection using rolling circle amplification.

Yasi Xing¹, Ping Wang, Yucui Zang

¹State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, 200050, China.

|May 9, 2013

Summary

A new colorimetric assay effectively detects H1N1 DNA using rolling circle amplification (RCA) and magnetic beads. This sensitive method offers a simple, cost-effective diagnostic tool for influenza A (H1N1) and other infectious diseases.

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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

Biotechnology
Molecular Diagnostics
Nanotechnology

Background:

Influenza A (H1N1) poses a significant public health threat.
Accurate and rapid diagnostic methods are crucial for disease control.
Existing detection methods may lack sensitivity or require complex procedures.

Purpose of the Study:

To develop a highly sensitive and specific colorimetric assay for H1N1 DNA detection.
To utilize rolling circle amplification (RCA) for signal amplification.
To create a simple, cost-effective, and visually detectable diagnostic platform.

Main Methods:

Designed specific oligonucleotide probes and a circular template for H1N1 DNA.
Immobilized probes onto magnetic beads (MBs) to create capture probes.
Employed rolling circle amplification (RCA) with phi29 polymerase for DNA amplification.
Utilized gold nanoparticles (AuNPs) for colorimetric detection via UV-vis spectroscopy.

Main Results:

Successfully developed a colorimetry-based RCA assay for H1N1 DNA detection.
Achieved a detection limit of 1 pmol L(-1) for H1N1 DNA.
Demonstrated a visible color change upon successful detection, observable by naked eye or spectroscopy.

Conclusions:

The developed RCA assay is highly sensitive and specific for H1N1 DNA detection.
The assay platform is convenient, simple, and cost-effective.
This method provides a novel diagnostic approach for H1N1 and potentially other infectious diseases.