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Updated: May 11, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Association between the interleukin 6 genotype at position -174 and atopic dermatitis
M Gharagozlou1, E Farhadi, M Khaledi
1Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Genetic variations in interleukin 6 (IL6) are linked to atopic dermatitis (AD). The G allele and GG genotype at position -174 of IL6 were more frequent in children with AD, suggesting increased cytokine production.
Area of Science:
- Genetics
- Immunology
- Dermatology
Background:
- Atopic dermatitis (AD) is a chronic skin condition with unknown causes, often appearing in infancy.
- Genetic and environmental factors are implicated in AD development.
Purpose of the Study:
- To investigate the association between interleukin 6 (IL6) gene polymorphisms and atopic dermatitis in Iranian children.
- To evaluate the frequency of IL6 alleles, genotypes, and haplotypes in AD patients compared to healthy controls.
Main Methods:
- Case-control study involving 89 Iranian children with AD and 139 healthy controls.
- Analysis of single-nucleotide polymorphisms (SNPs) in the IL6 gene at positions -174 and nt565.
- Genotyping and haplotype analysis to determine allele and genotype frequencies.
Main Results:
- The G allele at IL6 position -174 was significantly more frequent in AD patients (P < .001).
- The GG genotype at IL6 position -174 was significantly higher in AD patients (71%) compared to controls (30%) (P < .001).
- The GG haplotype (IL6 -174, nt565) was significantly more prevalent in the AD group (P < .001).
Conclusions:
- Increased frequency of the G allele and GG genotype at IL6 position -174 in AD patients suggests a genetic predisposition.
- Findings indicate potentially greater IL6 cytokine production in individuals with atopic dermatitis.
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