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Comparison of in-house developed ELISA with HPLC for measurement of hba1c

Saima Naz Mohsin1, Farkhanda Ghafoor, Muhammad Aasim

  • 1PMRC Research Centre, Shaikh Zayed Medical Complex, Lahore, Pakistan. saima_razi@hotmail.com

Journal of Immunoassay & Immunochemistry
|May 10, 2013
PubMed

In-house developed ELISA for HbA1c was evaluated in comparison with Bio-Rad D-10 Dual Program (HPLC). % HbA1c was estimated using said methods in 40 blood samples. Intra and inter assay coefficient of variation (CV) were in acceptable range i.e., <3%. HbA1c results obtained by two methods showed good correlation, (y = 0.8942x + 0.7229 R2 = 0.877). Sensitivity, specificity, positive, and negative predictive value for in-house ELISA classifying patients as controlled and uncontrolled diabetes against HPLC were recorded as 87%, 94.1%, 95.0%, and 93.8%, respectively, while accuracy of the test was 90.0%. In-house ELISA for HbA1c is not only accurate, cost effective and simple but also precise comparable to HPLC in deciding glycemic status of patients.

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