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Updated: May 11, 2026

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

Mitochondrial D-loop mutations and polymorphisms are connected with canine malignant cancers.

Brygida Slaska¹, Ludmila Grzybowska-Szatkowska, Magdalena Surdyka

¹Department of Biological Bases of Animal Production, University of Life Sciences in Lublin , Lublin , Poland .

Mitochondrial DNA

|May 10, 2013

Summary

Canine cancer research reveals significant D-loop sequence variations in mitochondrial DNA (mtDNA) between tumor, normal, and blood tissues. These polymorphic changes and heteroplasmy in dog tumors suggest mtDNA instability contributes to cancer development.

Keywords:

Cancer dog heteroplasmy mtDNA recombination

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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Area of Science:

Veterinary Oncology
Molecular Biology
Genetics

Background:

Mitochondrial DNA (mtDNA) mutations are implicated in various cancers.
The role of mtDNA D-loop sequence variations in canine carcinogenesis is not fully understood.

Purpose of the Study:

To investigate differences in the mtDNA D-loop nucleotide sequence between neoplastic, normal, and blood tissues in dogs.
To correlate these sequence variations with specific cancer types in canines.

Main Methods:

Analysis of D-loop nucleotide sequences from neoplastic tissue, normal tissue, and blood samples in dogs.
Identification and characterization of specific mutations, including T15620C.
Diagnosis of heteroplasmy in blood and tumor cells.

Main Results:

Substitutions in the D-loop sequence were detected in 62.5% of epithelial and 25% of mesenchymal tumors.
Two specific mutations (T15620C) were identified in epithelioma glandulae sebacei and carcinoma planoepithelialae keratodes.
Heteroplasmy was diagnosed in carcinoma planoepithelialae keratodes and "Comedo" carcinoma, suggesting potential mtDNA recombination.

Conclusions:

Polymorphic changes in the D-loop sequence appear to promote carcinogenesis in dogs.
The high prevalence of mtDNA mutations in canine tumors indicates genetic instability.
Heteroplasmy in blood and tumor cells, absent in normal tissue, may suggest a role for mtDNA recombination in canine cancer.