Improved EIS performance of an electrochemical cytosensor using three-dimensional architecture Au@BSA as sensing...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• Early tumor cell detection is crucial for effective cancer management.
• Existing methods for tumor cell detection often lack sensitivity and specificity.
• Development of advanced biosensors is needed for improved cancer diagnostics.

Purpose of the Study:

• To develop an ultrasensitive electrochemical cytosensor for quantitative determination of carcinoembryonic antigen (CEA)-positive tumor cells.
• To utilize three-dimensional (3D) architecture Au@BSA microspheres as a sensing layer.
• To conjugate monoclonal anti-CEA antibody (anti-CEA) for targeted cell detection.

Main Methods:

• Fabrication of Au@BSA microspheres as the sensing layer.
• Conjugation of anti-CEA antibodies for specific binding to CEA-positive cells.
• Utilizing electrochemical techniques (EIS, DPV, CV) for signal detection and analysis.
• Evaluating biocompatibility using MTT assay.

Main Results:

• The Au@BSA microspheres demonstrated excellent biocompatibility and electroconductivity.
• The cytosensor achieved a linear detection range from 5.2 × 10^1 to 5.2 × 10^7 cells/mL for CEA-positive cells.
• A low detection limit of 18 cells/mL was achieved.
• The sensor exhibited high specificity, good reproducibility (RSD of 3.5%), and stability.

Conclusions:

• The developed electrochemical cytosensor is a promising technique for early tumor cell monitoring.
• The 3D Au@BSA microsphere platform enhances detection sensitivity and specificity.
• This method provides a sensitive and reliable approach for quantitative determination of tumor cells.