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siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Small interfering RNAs (siRNA)

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Related Experiment Video

Updated: May 11, 2026

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

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Published on: June 2, 2022

Long double-stranded multiplex siRNAs for dual genes silencing.

Wei Peng¹, Jianxin Chen, Yinchao Qin

¹Biomics Biotechnologies Co., Ltd., Nantong, Jiangsu, China.

Nucleic Acid Therapeutics

|May 10, 2013

Summary

This study introduces novel multiplex small interfering RNAs (multi-siRNAs) capable of silencing multiple cancer-driving genes simultaneously. These engineered multi-siRNAs effectively inhibit bladder cancer cell proliferation by inducing apoptosis, offering a promising therapeutic strategy.

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MISSION esiRNA for RNAi Screening in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

Area of Science:

Molecular Biology
Cancer Therapeutics
RNA Interference

Background:

Simultaneous suppression of multiple oncogenes is a key strategy for effective cancer treatment.
Developing safe and effective RNA interference (RNAi) therapies requires overcoming challenges like immune response induction and off-target effects.

Purpose of the Study:

To design and evaluate novel long double-stranded multiplex small interfering RNAs (multi-siRNAs) for simultaneous dual gene silencing.
To investigate the role of a specific structural gap feature in multi-siRNAs for enhancing gene silencing and minimizing immune responses.
To assess the therapeutic potential of multi-siRNAs targeting SURVIVIN and BCL-2 in bladder cancer.

Main Methods:

Synthesis of long double-stranded multiplex small interfering RNAs (multi-siRNAs) with a designed gap feature.
Evaluation of gene silencing efficacy against SURVIVIN and BCL-2 oncogenes.
Assessment of immune response induction and impact on bladder cancer cell proliferation and apoptosis.

Main Results:

The designed gap feature in multi-siRNAs was essential for effective target gene silencing and avoidance of significant immune responses.
Multi-siRNAs demonstrated effective silencing of SURVIVIN and BCL-2.
Silencing led to increased caspase-3 mediated apoptosis and subsequent inhibition of bladder cancer cell proliferation.

Conclusions:

Rationally designed multi-siRNAs with a gap feature offer a promising approach for simultaneous oncogene suppression.
This strategy effectively inhibits bladder cancer cell proliferation through apoptosis induction.
The findings support the potential of these multi-siRNAs for future therapeutic applications in cancer treatment.