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Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

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Related Experiment Video

Updated: May 11, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

WNT3A signalling pathway in buffalo (Bubalus bubalis) embryonic stem cells.

Mohammad Zandi¹, Musharifa Muzaffar², Syed Mohmad Shah²

¹Department of Animal and Poultry Science and Fisheries, Agricultural Institute, Iranian Research Organisation for Science and Technology, Tehran 33535111, Iran.

Reproduction, Fertility, and Development

|May 10, 2013

Summary

WNT3A signaling is crucial for maintaining buffalo embryonic stem cells (ESCs) pluripotency and directing their differentiation. This pathway activates key genes, promoting cell proliferation and influencing cell structure formation.

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

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Published on: February 16, 2017

Related Experiment Videos

Last Updated: May 11, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Area of Science:

Stem Cell Biology
Developmental Biology
Molecular Signaling

Background:

Embryonic stem cells (ESCs) are vital for developmental studies and regenerative medicine.
Understanding the signaling pathways that regulate ESC pluripotency and differentiation is critical.
Buffalo ESCs offer a unique model for studying pluripotency in livestock.

Purpose of the Study:

To investigate the role of WNT3A signaling in maintaining buffalo ESC pluripotency.
To examine WNT3A's influence on buffalo ESC differentiation.
To analyze the WNT3A signaling pathway's conservation across different buffalo ESC derivation methods.

Main Methods:

Derivation of buffalo ESCs via in vitro fertilization (iESC), parthenogenesis (pESC), and cloning (cESC).
Quantitative real-time polymerase chain reaction (qRT-PCR) to assess gene expression.
Supplementation of culture media with WNT3A and analysis of downstream signaling (β-CATENIN).
Co-culture with fibroblast growth factor-2 (FGF2) and leukemia inhibitory factor (LIF).

Main Results:

WNT3A, its receptors, and pathway components were expressed in buffalo ESCs from all derivation methods.
WNT3A supplementation significantly increased β-CATENIN expression, confirming canonical WNT pathway activation.
WNT3A, alongside FGF2 and LIF, promoted undifferentiated buffalo ESC proliferation.
WNT3A inhibited neuronal differentiation and induced scaffold-like structure formation in buffalo ESCs.

Conclusions:

The WNT3A signaling pathway is conserved and essential for maintaining pluripotency in buffalo ESCs.
WNT3A plays a dual role, supporting both the maintenance of the undifferentiated state and directing differentiation pathways.
These findings provide insights into the molecular mechanisms governing buffalo ESC behavior.