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Updated: May 11, 2026

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens
Published on: June 23, 2022
Solution of the problem of nonspecific binding in solid-phase noninstrumental dot immunoassay
M S Bochkova1, V P Timganova, M B Raev
1Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia. krasnykh-m@mail.ru
Abstract:
In this work, we evaluated blocking properties of various reagents used in dot immunoassay test systems on nitrocellulose membranes employing carbon-based diagnosticum to determine antibodies to tetanus anatoxin, thermostable toxin of Yersinia pseudotuberculosis, and HIV-I. The results support the usage of a nonionic detergent Tween 20 which efficiently inhibited nonspecific protein binding to the solid-phase reagent surface and did not decrease sensitivity of the analysis.
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