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Updated: May 11, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Electrochemically-gated delivery of analyte bands in microfluidic devices using bipolar electrodes
Karen Scida1, Eoin Sheridan, Richard M Crooks
1Department of Chemistry and Biochemistry, Center for Electrochemistry, The University of Texas at Austin, Austin, TX 78712-0165, USA.
Abstract:
A method for controlling enrichment, separation, and delivery of analytes into different secondary microchannels using simple microfluidic architecture is described. The approach, which is based on bipolar electrochemistry, requires only easily fabricated electrodes and a low-voltage DC power supply: no pumps or valves are necessary. Upon application of a voltage between two driving electrodes, passive bipolar electrodes (BPEs) are activated that result in formation of a local electric field gradient. This gradient leads to separation and enrichment of a pair of fluorescent analytes within a primary microfluidic channel. Subsequently, other passive BPEs can be activated to deliver the enriched tracers to separate secondary microchannels. The principles and performance underpinning the method are described.
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