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Endogenous circulating DNA in systemic lupus erythematosus. Occurrence as multimeric complexes bound to histone
1Department of Medicine, State University of New York, Brooklyn 11203.
The Journal of Clinical Investigation
|July 1, 1990
Summary
Systemic lupus erythematosus (SLE) plasma DNA exists as multimeric complexes, potentially bound to histone. This suggests an oligonucleosomal (ON)-like structure, impacting lupus glomerulonephritis.
Area of Science:
- Molecular Biology
- Immunology
- Rheumatology
Background:
- Endogenous DNA in systemic lupus erythematosus (SLE) plasma is poorly understood.
- Plasma DNA is implicated as a precursor to DNA-containing immune complexes in lupus glomerulonephritis.
Purpose of the Study:
- To characterize the structure and properties of endogenous DNA circulating in SLE plasma.
- To investigate the potential association of SLE plasma DNA with histone.
Main Methods:
- Radiolabeling of DNA within whole SLE plasma.
- Polyacrylamide gel electrophoresis (PAGE) to analyze DNA size.
- Detergent treatment to disrupt DNA-protein complexes.
- Immunoprecipitation with anti-histone antibodies.
Main Results:
- SLE plasma DNA formed discrete bands on PAGE, resembling oligonucleosomal (ON) DNA ladders.
- Detergent treatment altered DNA migration, indicating DNA-protein complex formation.
- A significant portion of SLE plasma DNA immunoprecipitated with anti-histone antibodies, suggesting histone association.
- Immunoprecipitated DNA showed ON-like size distribution.
Conclusions:
- DNA in SLE plasma exists as multimeric complexes.
- At least a portion of this DNA is noncovalently bound to histone.
- These findings support an ON-like structure for SLE plasma DNA, with implications for its immunologic role in SLE.
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