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Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
The Ratio of X Chromosome to Autosomes02:45

The Ratio of X Chromosome to Autosomes

In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.  
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
The Y Chromosome Determines Maleness02:19

The Y Chromosome Determines Maleness

The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size. Today,...
Development of the Sexual Organs in the Embryo and Fetus01:15

Development of the Sexual Organs in the Embryo and Fetus

Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the male...
Dosage Compensation02:50

Dosage Compensation

In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with  distinct numbers of X chromosomes will have...

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Related Experiment Video

Updated: May 11, 2026

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
10:30

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Published on: January 20, 2014

Differential translation of Dazap1 transcripts during spermatogenesis.

Chi-Kai Yang1, Pauline Yen

  • 1Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.

Plos One
|May 10, 2013
PubMed
Summary

Deleted in AZoospermia Associated Protein 1 (DAZAP1) transcripts originate from alternative polyadenylation. DAZAP1-S translation is repressed with longer poly(A) tails, while DAZL regulates DAZAP1-L translation during spermatogenesis.

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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Published on: September 11, 2014

Related Experiment Videos

Last Updated: May 11, 2026

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
10:30

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Published on: January 20, 2014

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
09:48

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
08:35

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Published on: September 11, 2014

Area of Science:

  • Molecular Biology
  • Reproductive Biology
  • Gene Regulation

Background:

  • Deleted in AZoospermia Associated Protein 1 (DAZAP1) is crucial for spermatogenesis, with deficiency causing arrest.
  • DAZAP1 gene produces two transcripts, DAZAP1-L and DAZAP1-S, with suggested translational regulation during sperm development.

Purpose of the Study:

  • To determine the origin of DAZAP1-L and DAZAP1-S transcripts.
  • To investigate the differential translation of these two transcripts during spermatogenesis.

Main Methods:

  • Northern blot and 3' RACE analysis to identify transcript origins.
  • Sucrose gradient centrifugation to assess translational activity.
  • RNA pull-down assay coupled with mass spectrometry to identify interacting proteins.

Main Results:

  • Alternative polyadenylation generates the two DAZAP1 transcripts.
  • DAZAP1-S translation is repressed as spermatogenesis progresses, linked to poly(A) tail elongation.
  • DAZL binds to DAZAP1-L's 3'UTR and enhances its translation.

Conclusions:

  • The study elucidates the differential translational regulation of DAZAP1 transcripts.
  • Alternative polyadenylation and poly(A) tail length play key roles in controlling DAZAP1 expression during spermatogenesis.
  • DAZL acts as a specific regulator for DAZAP1-L translation.