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Updated: May 11, 2026
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Cost effective improvement in the protocol for detection of haemoglobin variants -a step forward in quality assurance
Natasha Ali1, Mohammad Khurshid
1Department of Pathology and Microbiology/Oncology, The Aga Khan University and Hospital, Stadium Road, P.O Box 3500, Karachi 74800, Pakistan. natasha.ali@aku.edu
Background:
We report the results of a cost effective improvement in the protocol for detection of haemoglobin variants which incorporates the findings of peripheral blood film along with the results of HPLC.
Findings:
A total of n = 10,844 samples were received from January 2011 till August 2011. Diagnosis of haemoglobinopathy was made in n = 1123 samples while other abnormalities included iron deficiency anaemia, megaloblastic anaemia, malarial parasite, autoimmune haemolytic anaemia and G6PD deficiency (n = 2473).
Conclusion:
We diagnosed 23% of abnormalities other than haemoglobinopathy by reviewing peripheral smear of samples received for detection of haemoglobin variants. This resulted in providing proper diagnosis to the referring physician without increment in cost.
