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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 11, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

[TALE nuclease engineering and targeted genome modification].

Yan Shen1, An Xiao, Peng Huang

  • 1College of Life Sciences, Peking University, Beijing, China. yshen@pku.edu.cn

Yi Chuan = Hereditas
|May 11, 2013
PubMed
Summary

Transcription activator-like effector nucleases (TALENs) offer a simpler, more specific, and less toxic method for targeted genome modification. This technology advances reverse genetics studies across various species, including zebrafish.

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Last Updated: May 11, 2026

Mouse Genome Engineering Using Designer Nucleases
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Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

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Published on: February 2, 2016

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • Engineered endonucleases (EENs) are revolutionizing reverse genetics.
  • Transcription activator-like effector nucleases (TALENs) present advantages over older technologies like zinc-finger nucleases (ZFNs).

Purpose:

  • To review recent advancements and future prospects of TALEN technology.
  • To highlight TALENs' structure, function, and construction strategies.
  • To document successful gene and species modifications using TALENs.

Summary:

  • TALENs enable precise DNA targeting for genome modification, offering improved specificity and reduced toxicity compared to ZFNs.
  • This review details TALEN construction, function, and application, providing a resource for researchers.
  • Successful TALEN-mediated modifications in various organisms, particularly zebrafish, are showcased.

Impact:

  • TALENs provide a powerful tool for reverse genetics, accelerating biological research.
  • The technology facilitates targeted genome editing in diverse species, expanding research possibilities.
  • Applications in zebrafish research demonstrate TALENs' potential for studying gene function and development.