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Related Experiment Videos

GP37 is different from filaggrin.

A Reano1, Y Sarret, J Thivolet

  • 1Laboratoire de Recherche Dermatologique et Immunologie, Hospital Edouard Herriot, Lyon, France.

The Journal of Investigative Dermatology
|July 1, 1990
PubMed
Summary
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Researchers investigated human epidermis differentiation markers, filaggrin and a 37 kD glycoprotein. They found the 37 kD band contains multiple markers, and the GP37 antibody targets the filaggrin precursor, not the glycoprotein itself.

Area of Science:

  • Dermatology
  • Biochemistry
  • Molecular Biology

Background:

  • Human epidermis differentiation involves specific protein markers.
  • Filaggrin and a 37 kD Concanavalin A (Con-A) reactive glycoprotein are key epidermal differentiation markers.
  • The precise identity and relationship between these markers require further elucidation.

Purpose of the Study:

  • To clarify the identity of filaggrin and a 37 kD Con-A reactive glycoprotein.
  • To determine if the 37 kD component is a single entity or a complex of markers.
  • To investigate the specificity of the anti-GP37 antibody in relation to filaggrin.

Main Methods:

  • Soluble proteins from human epidermis were extracted and separated using two-dimensional electrophoresis.
  • Con-A reactive glycoproteins were identified using iodinated lectin and autoradiography.

Related Experiment Videos

  • Filaggrin-related molecules were detected using monoclonal antibody AKH1 on nitrocellulose-transferred gels.

    • Main Results:

    • The 37 kD Con-A reactive component resolved into multiple glycoproteins via two-dimensional gel electrophoresis.
    • Concanavalin A (Con-A) did not bind to filaggrin.
    • The anti-GP37 serum did not identify any component in the epidermis extract but reacted similarly to AKH1 in human keratinocyte extracts, indicating specificity for the filaggrin precursor.

    Conclusions:

    • The 37 kD band is heterogeneous, containing distinct differentiation markers including filaggrin and Con-A reactive glycoproteins.
    • The GP37 antibody primarily recognizes the filaggrin precursor, not the mature glycoprotein.
    • This study refines the understanding of epidermal differentiation markers and antibody specificities.