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Updated: May 11, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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Published on: March 25, 2020

A mini-IRES sequence for stringent selection of high producers.

Jun Yan1, Hailin Yang, Guohua Yue

  • 1Institute of Surgery Research, State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute for Traffic Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, China.

Journal of Biosciences
|May 11, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new Internal Ribosome Entry Site (IRES) mutant, IRESmut3, which significantly reduces downstream gene expression. This allows for more efficient cellular resource allocation and stringent selection in biological applications.

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Last Updated: May 11, 2026

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Published on: October 7, 2021

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Expression Regulation

Background:

  • Internal Ribosome Entry Site (IRES) sequences enable co-expression of multiple proteins from a single mRNA.
  • Downstream genes often serve as reporters or selection markers, but their expression can be resource-intensive.
  • High expression of upstream genes may necessitate reduced downstream gene expression for efficiency and stringent selection.

Purpose of the Study:

  • To engineer an IRES mutant with significantly reduced co-translational efficiency.
  • To provide a tool for modulating downstream gene expression in bicistronic mRNA systems.
  • To facilitate resource economization and enhance selection stringency in high-level gene expression applications.

Main Methods:

  • Design and construction of a miniature IRES mutant (IRESmut3).
  • Evaluation of the co-translational efficiency of IRESmut3 in cellular systems.
  • Assessment of downstream gene expression levels mediated by IRESmut3.

Main Results:

  • The IRESmut3 mutant demonstrated dramatically diminished co-translational efficiency.
  • Reduced expression of downstream genes was observed when using IRESmut3.
  • The mutant sequence effectively modulates downstream gene expression as intended.

Conclusions:

  • IRESmut3 is an effective tool for reducing downstream gene expression from IRES elements.
  • This engineered IRES mutant offers a strategy for optimizing cellular resource use and improving selection stringency.
  • IRESmut3 provides a valuable advancement for bicistronic expression systems in biotechnology and molecular biology research.