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Related Concept Videos

Degenerative Disc Disease ll: Pathophysiology01:23

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...
Degenerative Disc Disease I: Introduction01:27

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...
Herniated Intervertebral Disc l: Introduction01:29

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

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Related Experiment Video

Updated: May 11, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

Genes associated with disc degeneration identified using microarray gene expression profiling and bioinformatics

Y Chen1, K Chen, M Li

  • 1Changhai Hospital, Second Military Medical University, Shanghai, China.

Genetics and Molecular Research : GMR
|May 11, 2013
PubMed
Summary
This summary is machine-generated.

Genetic therapies offer new hope for disc degeneration. Researchers identified key genes, MAP2K6 and RHOBTB2, that may be crucial targets for slowing or reversing this condition.

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Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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Related Experiment Videos

Last Updated: May 11, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.
05:46

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
14:33

Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species

Published on: August 30, 2012

Area of Science:

  • Biomedical research
  • Genetics
  • Molecular biology

Background:

  • Disc degeneration is a major cause of back and neck pain, significantly impacting quality of life and imposing economic burdens.
  • Current treatments are limited, highlighting the urgent need for novel therapeutic strategies like genetic interventions.

Purpose of the Study:

  • To identify key genes and pathways involved in disc degeneration using gene expression data.
  • To explore potential molecular targets for genetic therapies aimed at slowing or reversing disc degeneration.

Main Methods:

  • Downloaded and analyzed gene expression data from various stages of disc degeneration.
  • Identified differentially expressed genes (DEGs) by comparing degenerated discs with controls.
  • Constructed an interactive network of DEGs to elucidate their functional relationships.

Main Results:

  • Identified 2 significant DEGs between grade II and III discs, and 8 significant DEGs between grade II and IV discs.
  • Highlighted the potential roles of mitogen-activated protein family genes (e.g., MAP2K6) and Ras homologous (Rho) family genes (e.g., RHOBTB2) in disc degeneration progression.
  • MAP2K6 and RHOBTB2 emerged as potential specific therapeutic targets for advanced disc degeneration (grades III and IV).

Conclusions:

  • MAP2K6 and RHOBTB2 are identified as promising molecular targets for treating disc degeneration.
  • Further experimental validation is required to confirm the therapeutic potential of these identified genes.