[Influence of antibodies to DNA on MDCK cells and their intracellular localization]

Objects of the study were highly purified by liquid chromatography antibodies to DNA (IgG class) from the blood serums of healthy donors, patients with systemic lupus erythematosus (SLE) or rheumatoid arthritis (RA), and clinically healthy relatives of RA patients. It has been shown that, depending on the pathology, these antibodies differ in the DNA-hydrolyzing activity and differently affect the number of viable cells, morphology and chromatin of MDCK cells after co-culture. The antibodies to DNA were located in the area of nuclei. Biological role of the antibodies to DNA is discussed.