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Updated: May 11, 2026

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
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Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

Exploring neural cell dynamics with digital holographic microscopy.

P Marquet1, C Depeursinge, P J Magistretti

  • 1Centre de Neurosciences Psychiatriques, Centre Hospitalier Universitaire Vaudois (CHUV), Département de Psychiatrie, Site de Cery, CH-1008 Prilly/Lausanne, Switzerland.

Annual Review of Biomedical Engineering
|May 14, 2013
PubMed
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This summary is machine-generated.

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Digital holographic quantitative phase microscopy (DH-QPM) offers a reliable nanoscale imaging technique for cell biology. This method provides unique insights into cell states, dynamics, and neuronal activity.

Area of Science:

  • Biophysics
  • Cell Biology
  • Neurobiology
  • Optical Microscopy

Background:

  • Traditional cell imaging methods face limitations in providing quantitative nanoscale information.
  • Digital optics advancements have enabled new microscopy techniques.
  • Holographic microscopy offers label-free, quantitative phase imaging capabilities.

Purpose of the Study:

  • To review the development and applications of digital holographic quantitative phase microscopy (DH-QPM) for nanoscale cell imaging.
  • To highlight the biological information obtainable from quantitative phase signals.
  • To present novel DH-QPM applications in cell biology and neurobiology.

Main Methods:

  • Application of digital optics principles to holographic microscopy.

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Last Updated: May 11, 2026

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
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Published on: September 16, 2022

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Published on: March 31, 2022

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  • Development of a reliable and flexible DH-QPM technique.
  • Utilizing quantitative phase imaging for nanoscale analysis.
  • Employing a full digital holographic microscopy tomographic approach for neurobiology.

    • Main Results:

    • DH-QPM enables automated cell counting, recognition, classification, and 3D tracking.
    • The technique can discriminate between physiological and pathophysiological cell states and study nanoscale cell membrane fluctuations.
    • Original results demonstrate DH-QPM for multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics.

    Conclusions:

    • DH-QPM is a powerful, flexible technique for nanoscale cell imaging, offering unique biological insights.
    • Its applications span diverse areas of cell biology and neurobiology, addressing key research questions.
    • DH-QPM shows significant potential for advancing our understanding of cellular processes and neuronal function.