Related Experiment Video
Updated: May 11, 2026

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
Published on: March 24, 2023
Acute and subchronic dermal toxicity of Vitex negundo essential oil
Pronobesh Chattopadhyay1, Subham Banerjee, Manash Pratim Pathak
1Division of Pharmaceutical Technology and.
Abstract:
Vitex negundo is a common herb in different herbal formulation. The potential acute and sub-chronic dermal toxicities were evaluated as per OECD (Organization for Economic Cooperation and Development) guidelines 402 and 411, respectively. Both sexes of Wistar rats were exposed to Vitex negundo oil of 2000 mg/kg body weight for acute dermal toxicity, whereas in the dermal sub-chronic toxicity study, rats were exposed to Vitex negundo oil 250, 500 and 1000 mg/kg body weight, respectively, for five times a week for 90 d. In acute and sub-chronic toxicity studies, all animals were normal without any behavioral, serum biochemistry, hematology, necroscopical and histopathological changes. The no observed effect level (NOEL) and no observed adverse effect level (NOAEL) of Vitex negundo oil were 250 and 1000 mg/kg/day, respectively. Vitex negundo oil is under the category 5 (Unclassified) according to the Globally Harmonized System, with an LD50 value of over 2000 mg/kg.
Related Concept Videos
Toxicity Testing in Animals
Drug Toxicity: Allergic Reactions
Drug Toxicity: Dose-Dependent Reactions
Drug Toxicity: Overview
Drug Toxicity: Risk factors
Toxic Reactions: Overview
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
