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Related Experiment Video

Updated: May 11, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.

Thomas Gaj¹, Charles A Gersbach, Carlos F Barbas

¹The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA, USA.

Trends in Biotechnology

|May 14, 2013

Summary

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are powerful gene-editing tools. These nucleases enable precise DNA modifications for research, genetic analysis, and potential therapeutics.

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Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are engineered DNA-binding proteins fused to nucleases.
These chimeric nucleases offer programmable, sequence-specific DNA targeting capabilities.

Purpose of the Study:

To review the achievements and applications of ZFN and TALEN technologies in biological research.
To discuss the therapeutic potential and future prospects of site-specific nuclease technologies, including CRISPR/Cas systems.

Main Methods:

ZFNs and TALENs function by inducing targeted DNA double-strand breaks at specific genomic loci.
These breaks are repaired via nonhomologous end joining or homology-directed repair pathways, enabling genetic modifications.

Main Results:

ZFNs and TALENs have enabled a wide range of genetic modifications for research and analysis.
These technologies demonstrate significant potential for therapeutic applications in treating genetic disorders.

Conclusions:

Site-specific nuclease technologies like ZFNs and TALENs are revolutionizing genetic manipulation.
The field is rapidly advancing with the emergence of new tools such as CRISPR/Cas systems, promising broader applications.