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10:23
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
Published on: January 20, 2023
MUDENG is cleaved by caspase-3 during TRAIL-induced cell death
Jin Na Shin1, Ji Hye Han, Ji-Young Kim
1Department of Biochemistry, Chosun University School of Medicine, Gwang-Ju, Republic of Korea.
Biochemical and Biophysical Research Communications
|May 14, 2013
Summary
MUDENG (AP5M1) is processed by caspase-3 during TRAIL-induced cell death. This cleavage, occurring in its adaptin domain, reduces MUDENG
Area of Science:
- Molecular Biology
- Cell Death Pathways
- Apoptosis Research
Background:
- MUDENG (AP5M1) is an adaptin domain-containing gene linked to lymphoma cell death.
- The precise mechanism of MUDENG-mediated cell death remains largely uncharacterized.
Purpose of the Study:
- To investigate MUDENG protein alterations during Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced cell death.
- To elucidate the role of MUDENG processing in apoptosis.
Main Methods:
- Analysis of MUDENG processing in Jurkat and BJAB cells upon TRAIL stimulation.
- In vitro cleavage assays using recombinant active caspase proteins to confirm caspase-3 mediation.
- Identification of specific caspase cleavage sites within the MUDENG adaptin domain.
Main Results:
- MUDENG undergoes rapid processing in response to TRAIL, correlating with caspase activation.
- Caspase-3 was confirmed as the enzyme responsible for MUDENG cleavage.
- Identified caspase cleavage sites at D276 and D290 within the MUDENG adaptin domain.
- Cleaved MUDENG exhibited diminished cell-killing activity.
Conclusions:
- The adaptin domain of MUDENG is crucial for its cell death-inducing function.
- Caspase-3-mediated cleavage of MUDENG regulates its apoptotic activity.
- MUDENG processing is a key event in TRAIL-mediated apoptosis.
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