Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strengthening Health Research to Advance Equitable, Patient-Centered Care.

NEJM catalyst innovations in care delivery·2026
Same author

Ginsenoside Rg1 enhances stem cell abundance in aging human skeletal muscle after resistance exercise.

Journal of ginseng research·2026
Same author

Autofluorescence spectroscopy and multispectral autofluorescence microscopy for characterization of lupus nephritis in renal tissues.

Scientific reports·2026
Same author

Untrained Position-Encoded Multilayer Perceptron Network for Structured Illumination Microscopy Reconstruction.

Chemical & biomedical imaging·2026
Same author

ASO Visual Abstract: Do Postoperative Complications Impact Adjuvant Chemotherapy for Patients Undergoing Left-Side Pancreatectomy for Pancreatic Cancer?

Annals of surgical oncology·2026
Same author

Do Postoperative Complications Impact Adjuvant Chemotherapy in Patients Undergoing Left-Side Pancreatectomy for Pancreatic Cancer?

Annals of surgical oncology·2026

Related Experiment Video

Updated: May 11, 2026

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Quantitative analysis of autophagy using advanced 3D fluorescence microscopy.

Chun A Changou1, Deanna L Wolfson, Balpreet Singh Ahluwalia

  • 1Department of Biochemistry and Molecular Medicine, University of California, Davis, USA. cchangou@ucdavis.edu

Journal of Visualized Experiments : Jove
|May 14, 2013
PubMed
Summary

Prostate cancer cells treated with arginine deiminase (ADI) undergo autophagy. Researchers developed a 3D imaging method to track this process, aiding cancer therapy research.

More Related Videos

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
08:44

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Published on: August 3, 2018

Related Experiment Videos

Last Updated: May 11, 2026

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
08:44

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Published on: August 3, 2018

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Prostate cancer is a leading malignancy in men, with limited treatment options beyond surgery and hormone therapy.
  • Prostate tumor cells exhibit a metabolic deficiency, lacking argininosuccinate synthase (ASS), making them dependent on external arginine.
  • Arginine deiminase (ADI) depletes arginine, inducing metabolic stress and cell death in prostate cancer cells, including apoptosis and autophagy.

Purpose of the Study:

  • To investigate the role of autophagy in the death response of prostate cancer cells following ADI treatment.
  • To develop an experimental method for measuring the level and extent of autophagic response in cells.

Main Methods:

  • Developed a quantitative 3D fluorescence microscopy approach to track autophagy.
  • Utilized CWR22Rv1 prostate cancer cells labeled with fluorescent probes for autophagosomes and lysosomes.
  • Employed widefield deconvolution and super-resolution microscopy to capture early autophagy stages and image analysis for statistical data.

Main Results:

  • Successfully captured early stages of autophagy induction in CWR22Rv1 cells using 3D imaging.
  • Obtained quantitative data on autophagosome and lysosome number, size, distribution, and colocalization.
  • Demonstrated the ability to precisely track autophagy progress in living cells.

Conclusions:

  • The developed imaging-based approach enables precise tracking of autophagy in living cells.
  • This method facilitates further investigation into the role of autophagy in prostate cancer chemotherapy response.
  • Understanding autophagy's role is crucial for developing novel therapeutic strategies targeting metabolic vulnerabilities in cancer.