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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

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Metal-organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA.

Lifen Chen¹, Hanye Zheng, Xi Zhu

¹State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.

|May 14, 2013

Summary

A novel fluorescence sensor utilizes a metal-organic framework (MOF) for sensitive and selective detection of duplex DNA (ds-DNA). This cost-efficient sensor achieves a low detection limit, outperforming other platforms.

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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Published on: September 27, 2019

Area of Science:

Chemical Sensing
Biomolecular Detection
Materials Science

Background:

Developing sensitive and selective sensors for DNA detection is crucial for diagnostics.
Existing methods often face limitations in sensitivity, selectivity, or cost-efficiency.
Metal-organic frameworks (MOFs) offer unique properties for designing advanced sensing platforms.

Purpose of the Study:

To develop a simple, cost-efficient, sensitive, and selective fluorescence sensor for sequence-specific duplex DNA (ds-DNA) recognition.
To utilize a metal-organic framework (MOF) as the sensing platform for enhanced performance.
To demonstrate the sensor's capability for detecting specific DNA sequences in vitro.

Main Methods:

A metal-organic framework (MOF), N,N-Bis(2-hydroxy-ethyl)dithiooxamidatocopper(II) (H(2)dtoaCu), was employed as the sensing platform.
A dye-labeled triplex-forming oligonucleotide (TFO) probe was used, which is chemisorbed by the MOF and quenches fluorescence.
Sequence-specific interaction between the TFO and target ds-DNA (HIV RNA PPT sequence) triggers fluorescence recovery.

Main Results:

The developed sensor demonstrated sequence-specific recognition of ds-DNA.
Fluorescence recovery was observed upon the interaction of TFO with the target ds-DNA, forming a triplex structure.
A low detection limit of 1.3 nmol L(-1) (S/N = 3) was achieved with good selectivity.
The sensor's performance surpassed that of graphene oxide platforms and electrochemical-DNA sensors.

Conclusions:

A simple, cost-efficient, sensitive, and selective fluorescence sensor for ds-DNA detection has been successfully developed using an MOF platform.
The sensor leverages the specific interaction between TFO and ds-DNA to induce a measurable fluorescence signal.
This MOF-based sensor represents a promising advancement in DNA detection technology, offering superior performance compared to existing methods.