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An easy method for impaling cells using the micromanipulation technique.

A L Perez-Samartin1, L Martinez-Millan, F Doñate-Oliver

  • 1Department of Neurosciences, Universidad del País Vasco, Leioa, Spain.

Journal of Neuroscience Methods
|May 1, 1990
PubMed
Summary
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This study introduces a novel microscope design for easier neuron filling. The simultaneous displacement of the microscope head and micromanipulator simplifies intracellular iontophoresis injection of Lucifer yellow in brain slices.

Area of Science:

  • Neuroscience
  • Microscopy techniques
  • Cell biology

Background:

  • Intracellular injection techniques are crucial for neuroscience research.
  • Filling neurons, particularly in brain slices, presents technical challenges.
  • Current methods may lack precision or ease of use.

Purpose of the Study:

  • To describe a new method for improving intracellular injection.
  • To facilitate the filling of neurons in brain slices using iontophoresis.
  • To enhance the ease and efficiency of Lucifer yellow filling.

Main Methods:

  • A fixed-stage microscope with an attached micromanipulator was utilized.
  • Simultaneous displacement of the microscope head and micromanipulator during focusing was implemented.

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  • Axial displacement of the microscope towards the target cell was enabled.

    • Main Results:

    • The described method simplifies the process of neuron filling.
    • Intracellular iontophoresis injection of Lucifer yellow into neurons in brain slices became considerably easier.
    • The simultaneous movement design enhances precision and accessibility.

    Conclusions:

    • The novel microscope design offers a significant improvement for neuron injection.
    • This technique facilitates detailed study of neuronal morphology and function.
    • The method is particularly beneficial for experiments requiring precise intracellular labeling.