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Related Experiment Video

Updated: May 11, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Sequence-based mapping of the polyploid wheat genome.

Cyrille Saintenac1, Dayou Jiang, Shichen Wang

  • 1Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66502, USA.

G3 (Bethesda, Md.)
|May 14, 2013
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing enabled high-resolution gene mapping in wheat by developing a comprehensive reference map. This resource aids in understanding genetic variation and linking physical and genetic wheat genome maps.

Keywords:
contig anchoringgene mappingreference mapsequence-based genotyping

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Area of Science:

  • Genomics
  • Plant genetics
  • Bioinformatics

Background:

  • Wheat's complex polyploid genome poses challenges for genetic variation studies.
  • New sequencing technologies offer potential for high-resolution genome analysis.

Purpose of the Study:

  • To apply next-generation sequencing for high-resolution gene mapping in wheat.
  • To develop a bioinformatical pipeline for variant analysis in polyploid wheat.
  • To create a high-density reference map for the wheat genome.

Main Methods:

  • Utilized next-generation sequencing on a wheat doubled-haploid mapping population.
  • Developed a bioinformatical pipeline for variant analysis in polyploid wheat.
  • Integrated multiple marker types (SNP, DArT, SSR/STS, GBS) to construct a reference map.

Main Results:

  • A bioinformatical pipeline for variant analysis in polyploid wheat was successfully developed.
  • The variant mapping results aligned with those from the wheat 9000 SNP iSelect assay.
  • A comprehensive reference map integrating over 400,000 markers was constructed, enabling gene mapping within 40-100 kb of genes.
  • Gene loci for seed color were mapped, with one marker located within the red seed color gene.

Conclusions:

  • The developed high-density reference map is a valuable resource for gene mapping in wheat.
  • This map facilitates the linkage of physical and genetic maps of the wheat genome.
  • The study demonstrates the utility of next-generation sequencing for high-resolution genetic studies in complex plant genomes.