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Electroporation of Craniofacial Mesenchyme

Electroporation of Craniofacial Mesenchyme

Published on: November 28, 2011

A method for electroporation to study gene function in mammary gland development.

Jong-Min Lee¹, Eun-Jung Kim, Han-Sung Jung

¹Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 project, Oral Science Research Institute, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.

Journal of Mammary Gland Biology and Neoplasia

|May 14, 2013

Summary

Gene manipulation via electroporation and in vitro embryo culture is crucial for understanding embryonic development. This protocol details methods for studying mammary gland development, aiding gene function analysis.

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Area of Science:

Developmental biology
Molecular genetics

Background:

Gene function is critical for understanding embryonic development and signaling networks.
Electroporation and in vitro embryo culture are key techniques for introducing foreign genes and studying their effects.
Trowell culture offers a stable and user-friendly method for mammalian embryo culture, particularly for mammary gland development studies.

Purpose of the Study:

To provide a detailed protocol for gene manipulation using electroporation combined with in vitro embryo culture.
To facilitate the functional analysis of specific genes during embryonic mammary gland development.

Main Methods:

Electroporation for gene introduction into embryos.
In vitro embryo culture using the Trowell technique.
Detailed step-by-step protocol for both techniques.

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Last Updated: May 11, 2026

Electroporation of Craniofacial Mesenchyme

Electroporation of Craniofacial Mesenchyme

Published on: November 28, 2011

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Published on: February 10, 2023

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Published on: August 3, 2012

Main Results:

The protocol enables precise manipulation of gene expression in embryonic cells.
It allows for direct observation of gene function and downstream signaling in developing mammary glands.
Successful application of electroporation and Trowell culture for embryonic mammary gland research.

Conclusions:

This protocol serves as a valuable resource for researchers studying embryonic development.
It enhances the ability to investigate gene function and signaling pathways in mammalian embryogenesis.
The combined techniques are essential for advancing the field of developmental biology and mammary gland research.