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Related Experiment Video

Updated: May 11, 2026

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

High-throughput tetrad analysis.

Catherine L Ludlow1, Adrian C Scott, Gareth A Cromie

  • 1Institute for Systems Biology, Seattle, Washington, USA.

Nature Methods
|May 14, 2013
PubMed
Summary
This summary is machine-generated.

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Barcode-enabled sequencing of tetrads (BEST) streamlines genetic analysis by enabling high-throughput tetrad isolation and genotyping. This method significantly accelerates genetic mapping studies in yeast and other microorganisms.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Tetrad analysis is a crucial genetic technique but is limited by manual labor and low throughput.
  • Current methods hinder integration with high-throughput DNA sequencing.

Purpose of the Study:

  • To develop a rapid, high-throughput method for tetrad analysis.
  • To enable accurate inference of genotypes and genetic markers.

Main Methods:

  • Utilized a meiosis-specific GFP fusion protein for Fluorescence-Activated Cell Sorting (FACS) isolation of tetrads.
  • Incorporated molecular barcodes for identifying spores from the same tetrad during genotyping.

Main Results:

  • Developed barcode-enabled sequencing of tetrads (BEST) for high-throughput analysis.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Related Experiment Videos

Last Updated: May 11, 2026

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

  • Achieved isolation of over 3,000 yeast tetrads in 3 hours, a significant increase in efficiency.
  • Enabled accurate inference of missing genetic markers and full genotypes.

    • Conclusions:

    • BEST significantly enhances the speed and scale of tetrad analysis.
    • The method is transferable to other microorganisms, simplifying laborious meiotic mapping.
    • BEST facilitates integration with high-throughput sequencing for advanced genetic studies.