Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Negative association between asthma and tuberculosis: the mediating effect of albumin.

The Journal of asthma : official journal of the Association for the Care of Asthma·2026

Same author

Paradox of atropine in myopia control: exploring dose-dependent efficacy, rebound effects, and optimal risk-benefit balance-a perspective.

Frontiers in public health·2026

Same author

Bimetallic valence relay catalysis in EuTb/N-doped carbon nanosheets: temporal decoupling of oxygen reduction resolves competitive kinetics for ultrasensitive electrochemiluminescence detection of L-cysteine.

Mikrochimica acta·2026

Same author

PRMT5-Dependent Stabilization of VPS34 Orchestrates Copper Trafficking to Shield Cancer Cells from Cuproptosis and Radiotherapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

mTORC2 Phosphorylation of GSDME-N Drives Cullin4B-Mediated Proteasomal Degradation to Suppress Pyroptosis and Confer Radioresistance in Small Cell Lung Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Polyamine Metabolism and the DHPS/eIF5A Hypusination Axis: From Metabolic Reprogramming to a Therapeutic Achilles' Heel in Melanoma.

Biomolecules·2026

Search research articles

Related Experiment Video

Updated: May 11, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

EBER in situ hybridization for Epstein-Barr virus.

Lawrence M Weiss¹, Yuan-Yuan Chen

¹Clarient Pathology Services, Inc., Aliso Viejo, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2013

Summary

Epstein-Barr virus (EBV) is detected in tissues using Epstein-Barr encoding region (EBER) in situ hybridization. This method identifies EBV in infected cell nuclei, with RNA degradation being a common cause of false negatives.

Area of Science:

Virology
Molecular Biology
Pathology

Background:

Epstein-Barr virus (EBV) is a common human herpesvirus.
Latent EBV infection is associated with various diseases.
Accurate detection of EBV in tissues is crucial for diagnosis and research.

Purpose of the Study:

To describe the methodology and interpretation of Epstein-Barr encoding region (EBER) in situ hybridization for EBV detection.
To highlight the advantages of non-isotopic methods for EBER detection.
To identify common pitfalls and quality control measures for EBER in situ hybridization.

Main Methods:

Epstein-Barr encoding region (EBER) in situ hybridization utilizes non-isotopic probes.
Detection involves identifying EBER RNA within the nuclei of infected cells.

More Related Videos

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Related Experiment Videos

Last Updated: May 11, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

A polyT probe serves as a control for RNA preservation in tissue sections.

Main Results:

Positive EBER in situ hybridization shows distinct nuclear staining in EBV-infected cells.
Staining accentuates chromatin and typically excludes the nucleolus.
False-negative results are often due to RNA degradation, identifiable with a polyT control.

Conclusions:

EBER in situ hybridization is a reliable method for detecting EBV in tissue samples.
Proper tissue handling and RNA quality control are essential for accurate results.
This technique aids in the diagnosis and understanding of EBV-associated conditions.