Related Experiment Video
Updated: May 11, 2026
09:14
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
Epstein-Barr virus (EBV) load determination using real-time quantitative polymerase chain reaction
Hongxin Fan1, Ryan S Robetorye
1Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2013
Summary
Epstein-Barr virus (EBV) infection is widespread and linked to cancers. This study details a sensitive RQ-PCR assay for quantifying EBV DNA in patient samples for improved diagnosis and monitoring.
Area of Science:
- Virology
- Molecular Biology
- Oncology
Background:
- Epstein-Barr virus (EBV) establishes lifelong infections in most humans.
- EBV is associated with various cancers, including lymphomas, carcinomas, and sarcomas.
- EBV-linked lymphoproliferative disorders are common in immunocompromised individuals.
Purpose of the Study:
- To describe a laboratory-developed real-time quantitative polymerase chain reaction (RQ-PCR) assay for Epstein-Barr virus (EBV) viral load determination.
- To validate the use of RQ-PCR for detecting EBV DNA in clinical samples.
Main Methods:
- Real-time quantitative polymerase chain reaction (RQ-PCR) assay.
- Detection of EBV DNA in cell-free plasma and cerebrospinal fluid (CSF).
Main Results:
- RQ-PCR is a fast, sensitive, and accurate method for EBV DNA quantification.
- The assay requires minimal nucleic acid input and is relatively simple to perform.
- The described method is suitable for clinical applications in EBV diagnostics.
Conclusions:
- RQ-PCR is the preferred method for EBV viral load assessment.
- The developed assay enables reliable detection of EBV DNA in plasma and CSF.
- Accurate EBV viral load monitoring is crucial for managing EBV-associated diseases.
