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[Synthesis and identification of rutin complete antigen and analysis its immunogenicity]
Xiao-Yan Bao1, Fu-Yuan He, Jiao-Li Zeng
1Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.
Objective:
Synthesis and identification of complete antigen of rutin, the traditional Chinese medicine active ingredient, and develop rapid detection of rutin using enzyme-linked immunoassay method (ELISA). Immunogenicity of the complete antigen was also studied.
Method:
Prepare the complete antigen by sodium periodate solution and identified by UV scanning and SDS-PAGE test. Male New Zealand white rabbits were immunized by the antigen to obtain the antiserum.
Result:
The results of UV analysis showed that the coupling ratio of complete antigen is 13: 1. SDS-PAGE display of the artificial antigen was delayed compared with bovine serum protein. The titer of rutin antibody is 1:4 000. The sensitivity of IC50 was 5.37 mg x L(-1), the lowest detection limit was 1 mg x L(-1), the average recovery was 102%, the intra and interspecific RSD were less than 10%, cross-reactivity rate of antibodies and other analogs were less than 1%.
Conclusion:
Rutin complete antigen was synthesized successfully, and the rapid detection of rutin by ELISA method was successfully established.
