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Updated: May 11, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae
Masatomo Morita1, Shouji Yamamoto, Hirotaka Hiyoshi
1Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. mmorita@niid.go.jp
Abstract:
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS-related genes were distributed among the various serogroups and pulsed-field gel electrophoresis of NotI-digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS-related genes had similar patterns. Additionally, naturally competent T3SS-negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS-related genes occurs among V. cholerae in natural ecosystems.
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