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Related Experiment Video

Updated: May 11, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
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Published on: July 3, 2013

BRCA1 and p53 regulate critical prostate cancer pathways.

P De Luca1, C P Moiola, F Zalazar

  • 1Department of Biological Chemistry, School of Sciences (FCEN), University of Buenos Aires (UBA), IQUIBICEN-CONICET, Buenos Aires, Argentina.

Prostate Cancer and Prostatic Diseases
|May 15, 2013
PubMed
Summary

BRCA1 and p53 play crucial roles in prostate cancer (PCa) therapy response. While BRCA1 induces GADD153 independently, p53 abolishes this induction upon DNA damage, impacting PCa cell aggressiveness and treatment outcomes.

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Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 17, 2011

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • BRCA1 gene mutations increase breast, ovarian, and prostate cancer (PCa) risks.
  • BRCA1 protein targets GADD153, enhancing doxorubicin sensitivity in PCa cells.
  • p53 is a critical target in cancer therapy, prompting investigation into its role in GADD153 regulation.

Purpose of the Study:

  • To investigate the role of p53 in regulating GADD153 transcription.
  • To understand the interplay between BRCA1, p53, and GADD153 in prostate cancer cells.
  • To identify key genes modulated by BRCA1 and p53 in PCa.

Main Methods:

  • Quantitative PCR (RT-qPCR) and Western blot analyses.
  • Luciferase assays and chromatin immunoprecipitation-qPCR.
  • In vitro studies in PCa cell lines and in vivo xenograft models.

Main Results:

  • GADD153 induction by doxorubicin was abolished in p53-expressing cells.
  • BRCA1 protein associates with the GADD153 promoter in a p53-dependent manner after DNA damage.
  • BRCA1 and p53 modulate genes involved in DNA damage, cell cycle, EMT, and Hedgehog pathways.
  • BRCA1 knockdown in vivo increased PCa tumor growth.

Conclusions:

  • p53 abolishes GADD153 induction by DNA damage, despite BRCA1's independent induction.
  • BRCA1 and p53 collaboratively regulate critical PCa-associated genes.
  • These findings offer insights into PCa therapy response mechanisms.