No factor left behind: generation of transgene-free induced pluripotent stem cells
Mo Li1, Juan Carlos Izpisua Belmonte
1Salk Institute for Biological Studies 10010 N. Torrey Pines Rd., La Jolla, CA USA.
Abstract:
Induced pluripotent stem cells (iPSCs) hold great potential for regenerative medicine, yet their implementation in the clinic still seems far out of reach due to concerns about their safety. The development of safe and efficient reprogramming methods is a critical step towards clinical application of iPSCs. Recently, much progress has been made in the technology of generating iPSCs that are free of integrated transgenes. The newly developed methods improve genome integrity of iPSCs and will likely replace virus-based reprogramming in the future. Here, we review the recent technological advancements of transgene-free reprogramming and discuss the challenges of ensuring genome integrity of iPSCs.
More Related Videos
Related Concept Videos
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic cells are...
Somatic to iPS Cell Reprogramming
Stem Cell Culture


