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Updated: May 11, 2026

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Published on: August 3, 2017

[Research progress in aromatic prenyltransferases originated from microorganisms].

Xiao Liu¹, Ri-Dao Chen, Dan Xie

¹State Key Laboratory ofBioactive Substance and Function of Natural Medicines, Institute ofMateria Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.

Yao Xue Xue Bao = Acta Pharmaceutica Sinica

|May 16, 2013

Summary

Prenylation of aromatic compounds is vital for natural product diversity and bioactivity. Microbial aromatic prenyltransferases offer promising chemoenzymatic synthesis routes for these valuable molecules.

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Area of Science:

Biochemistry and Natural Product Chemistry
Enzymology and Metabolic Engineering

Context:

Prenylation of aromatic compounds is crucial for generating diverse natural products with enhanced bioactivity and bioavailability.
Low natural abundance and synthetic challenges limit the study of prenylated aromatic compounds.
Microbial enzymes, specifically aromatic prenyltransferases, are key targets for overcoming these limitations.

Purpose:

To review the classification, structural characteristics, and catalytic mechanisms of microbial aromatic prenyltransferases.
To highlight the potential of these enzymes in the chemoenzymatic synthesis of prenylated natural products.
To provide an overview of recent advancements in the field of microbial aromatic prenyltransferases.

Summary:

This review consolidates current knowledge on aromatic prenyltransferases from microorganisms.
It covers their classification, structural features, enzymatic functions, and recent research progress.
The focus is on their utility in synthesizing valuable prenylated aromatic compounds.

Impact:

Facilitates the exploration and utilization of prenylated natural products in various applications.
Provides a foundation for enzyme engineering and directed evolution of aromatic prenyltransferases.
Enables the development of sustainable and efficient biocatalytic methods for natural product synthesis.